منابع مشابه
Polyamine uptake in carrot cell cultures.
Putrescine and spermidine uptake into carrot (Daucus carota L.) cells in culture was studied. The time course of uptake showed that the two polyamines were very quickly transported into the cells, reaching a maximum absorption within 1 minute. Increasing external polyamine concentrations up to 100 millimolar showed the existence of a biphasic system with different affinities at low and high pol...
متن کاملEffects of polyamine biosynthesis inhibitors on S-adenosylmethionine synthet- ase and S-adenosylmethionine decarboxylase activities in carrot cell cultures
Changes in the activites o f S-adcnosylmethionine ( S A M ) synthetase (methionine adenosyltransferase, EC 2.5.1.6.) and SAM decarboxylase (EC 4.1.1.50) werc studied in carrot (Daucus carota) cell cultures in response to 2,4-dichlorophenoxyacetic acid (2,4-D) and several inhibitors o f polyamine biosynthesis. Activity o f SAM synthetase increased significantly during the first 5 days o f cultur...
متن کاملProteoglycan involvement in polyamine uptake.
We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate (Ki=0.16+/-0.04 microM). Treatment of fibroblasts with either heparan sulphate lyase, p-nitrophenyl-O-beta-D-xylopyranoside or chlorate reduced spermine uptake consid...
متن کاملAnalysis of the rolC Promoter Region lnvolved in Somatic Embryogenesis-Related Activation in Carrot Cell Cultures'
In cell cultures of carrot (Daucus carota l.), somatic embryogenesis can be induced by transferring cells from a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) to one devoid of 2,4-D. Previous analysis of transgenic carrot cells containing the 5' noncoding sequence of the Ri plasmid rolC and a structural gene for bacterial 0-glucuronidase (uidA) has shown that the chimeric gene is act...
متن کاملAnalysis of the rolC promoter region involved in somatic embryogenesis-related activation in carrot cell cultures.
In cell cultures of carrot (Daucus carota L.), somatic embryogenesis can be induced by transferring cells from a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) to one devoid of 2,4-D. Previous analysis of transgenic carrot cells containing the 5' non-coding sequence of the Ri plasmid rolC and a structural gene for bacterial beta-glucuronidase (uidA) has shown that the chimeric gene is...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1987
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.84.2.374